The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 09, 2018

Filed:

May. 23, 2012
Applicants:

Akira Wada, Saitama, JP;

Hiroyuki Osada, Saitama, JP;

Inventors:

Akira Wada, Saitama, JP;

Hiroyuki Osada, Saitama, JP;

Assignee:

RIKEN, Saitama, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/16 (2006.01); C07H 21/04 (2006.01); C40B 30/04 (2006.01); C12N 15/00 (2006.01); C12P 21/06 (2006.01); G01N 33/53 (2006.01); C07K 17/10 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5308 (2013.01); C07K 17/10 (2013.01); C12N 15/1041 (2013.01); C07H 21/04 (2013.01); C07K 2319/80 (2013.01); C07K 2319/85 (2013.01); C12N 15/1034 (2013.01); C12N 2800/00 (2013.01);
Abstract

Using a nucleic acid construct, association of a polypeptide with a sequence coding therefor and screening of a polypeptide that binds to a target substance are carried out, which nucleic acid construct comprises a 5'-untranslated region and a coding region, wherein the above-mentioned coding region comprises a sequence coding for a polypeptide subjected to be displayed, a sequence coding for a first nucleic acid binding polypeptide, and a sequence coding for a second nucleic acid binding polypeptide; the above-mentioned 5′-untranslated region comprises a first sequence capable of binding to a first nucleic acid binding polypeptide and a second sequence capable of binding to second nucleic acid binding polypeptide; and, when the above-mentioned nucleic acid construct is introduced in a translation system, a fusion protein translated from the coding region of the above-mentioned nucleic acid construct forms a complex with an RNA corresponding to the above-mentioned nucleic acid construct.


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