The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 09, 2018

Filed:

Jul. 12, 2013
Applicant:

Kabushiki Kaisha Dnaform, Yokohama-shi, Kanagawa, JP;

Inventors:

Yoshihide Hayashizaki, Tokyo, JP;

Takeshi Hanami, Yokohama, JP;

Takahiro Soma, Tokyo, JP;

Yasumasa Kimura, Yokohama, JP;

Hajime Kanamori, Yokohama, JP;

Yasumasa Mitani, Yokohama, JP;

Assignee:

KABUSHIKI KAISHA DNAFORM, Kanagawa, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6818 (2013.01); C12Q 1/6827 (2013.01); C12N 2330/30 (2013.01);
Abstract

The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.


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