The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 02, 2018

Filed:

Nov. 18, 2014
Applicant:

Meridian Bioscience, Inc., Cincinnati, OH (US);

Inventors:

Vladimir I Slepnev, Cincinnati, OH (US);

Ahmer Kodvawala, Cincinnati, OH (US);

Brian Loeffler, Cincinnati, OH (US);

Anand Hindupur, Cincinnati, OH (US);

Reddy Ponaka, Cincinnati, OH (US);

Vecheslav A. Elagin, Cincinnati, OH (US);

Assignee:

Meridian Bioscience, Inc., Cleveland, OH (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12Q 1/6806 (2013.01); C12Q 2527/125 (2013.01); C12Q 2527/137 (2013.01);
Abstract

The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.


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Slepnev, Vladimir. (2018). Methods of nucleic acid fractionation and detection (U.S. Patent No. 9856514). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9856514

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