The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 02, 2018

Filed:

May. 26, 2015
Applicant:

Jiangnan University, Wuxi, CN;

Inventors:

Zhouping Wang, Wuxi, CN;

Xiujuan Chen, Wuxi, CN;

Huajie Gu, Wuxi, JP;

Yu Xia, Wuxi, CN;

Shijia Wu, Wuxi, CN;

Nuo Duan, Wuxi, CN;

Xiaoyuan Ma, Wuxi, CN;

Assignee:
Attorney:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 15/115 (2010.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
C12N 15/115 (2013.01); G01N 33/5308 (2013.01); C12N 2310/16 (2013.01); C12N 2320/13 (2013.01);
Abstract

This invention provides a small molecule oligonucleotide aptamer binding to T-2 toxin specifically, and the sequence of the ssDNA aptamer is SEQ ID NO: 1. Through the graphene oxide separation-based Systematic Evolution of Ligands by Exponential Enrichment, single-stranded oligonucleotide aptamer with high affinity and specific identification of T-2 toxin was obtained in vitro selection. The aptamer has a broad application prospect, which can be used for separation and enrichment of trace T-2 toxin in the sample. It can also be used as a reporter aptamer for the detection of T-2 toxin in food by means of functional groups labeling and other means.


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