The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 26, 2017
Filed:
Feb. 26, 2010
Noha Ahmed Mousa, Assiut, EG;
Mais J. Jebrail, Richmond Hill, CA;
Mohamed Omar Abdelgawad, Assiut, EG;
Aaron R. Wheeler, Toronto, CA;
Robert Fredric Joseph Casper, Toronto, CA;
Noha Ahmed Mousa, Assiut, EG;
Mais J. Jebrail, Richmond Hill, CA;
Mohamed Omar Abdelgawad, Assiut, EG;
Aaron R. Wheeler, Toronto, CA;
Robert Fredric Joseph Casper, Toronto, CA;
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO, Toronto, Ontario, CA;
Abstract
Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.