The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 26, 2017

Filed:

Nov. 13, 2014
Applicant:

Illumina, Inc., San Diego, CA (US);

Inventors:

Arnold Oliphant, Sunnyvale, CA (US);

John R. Stuelpnagel, Santa Barbara, CA (US);

Mark S. Chee, Encinitas, CA (US);

Scott L. Butler, Sandwich, GB;

Jian-Bing Fan, San Diego, CA (US);

Min-Jui Richard Shen, Poway, CA (US);

Assignee:

Illumina, Inc., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6876 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6837 (2013.01); C12Q 2600/16 (2013.01); Y10T 436/143333 (2015.01);
Abstract

A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.


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