The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 26, 2017

Filed:

Jan. 22, 2014
Applicant:

Anp Technologies, Inc., Newark, DE (US);

Inventors:

Yli Remo Vallejo, Jr., Newark, DE (US);

David Milunic, Newark, DE (US);

Ray Yin, Newark, DE (US);

Renee Street, Bel Air, MD (US);

De Chen, Newark, DE (US);

Assignee:

ANP Technologies, Inc., Newark, DE (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/46 (2006.01); G01N 33/558 (2006.01); C12Q 1/26 (2006.01); C12Q 1/42 (2006.01); C12Q 1/44 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/46 (2013.01); C12Q 1/26 (2013.01); C12Q 1/42 (2013.01); C12Q 1/44 (2013.01); C12Y 301/01001 (2013.01); C12Y 301/01007 (2013.01); G01N 33/558 (2013.01); G01N 2333/916 (2013.01);
Abstract

A device and method for the rapid on-site detection of inhibitors of enzymes, such as acetylcholinesterase, is described wherein the device contains all reagents added to a sample pad containing dried releasable enzyme creating a reaction mixture wherein inhibitor deactivates the enzyme, while said reaction mixture travels via a longitudinal membrane to a distal porous pad containing a substrate for the enzyme. The reaction of the enzyme and the substrate results in a product that can generate a measurable signal such as color, fluorescence or luminescence to serve as a reporter. Signal that is generated at this reaction zone is inversely proportional to inhibitor concentration in the test sample. A device containing two such strips, one for a test sample, the other for a negative control fluid as an onboard comparator is described. A purpose-built reader or an illuminating device, such as, containing an incandescent light source, a diode, a UV light source or any other illumination source that is suitable for the reporter or mere visualization is used to determine the level of reporter.


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