The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 26, 2017

Filed:

Jun. 30, 2014
Applicant:

Kansas State University Research Foundation, Manhattan, KS (US);

Inventors:

Jun Li, Manhattan, KS (US);

Duy Hua, Manhattan, KS (US);

Lateef Uddin Syed, Portland, OR (US);

Allan Prior, Manhattan, KS (US);

Luxi Swisher, Manhattan, KS (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/06 (2006.01); G01N 33/00 (2006.01); G01N 33/48 (2006.01); C12Q 1/37 (2006.01); B01J 19/00 (2006.01); G01N 27/27 (2006.01); G01N 27/30 (2006.01); B82Y 15/00 (2011.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); B01J 19/0046 (2013.01); G01N 27/27 (2013.01); G01N 27/308 (2013.01); B01J 2219/00653 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00725 (2013.01); B82Y 15/00 (2013.01); G01N 2333/96466 (2013.01); G01N 2800/7028 (2013.01);
Abstract

An electrochemical method for measuring the activity of enzymes using nanoelectrode arrays fabricated with vertically aligned carbon nanofibers. Short peptide substrates specific to disease-related enzymes are covalently attached to the exposed nanofiber tips. A redox moiety, such as ferrocene, can be linked at the distal end of the nanofibers. Contact of the arrays with a biological sample containing one or more target enzymes results in cleavage of the peptides and changes the redox signal of the redox moiety indicating the presence of the target enzymes.


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