The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 26, 2017
Filed:
Apr. 17, 2013
Applicant:
Greenovation Biotech Gmbh, Freiburg, DE;
Inventors:
Wolfgang Jost, Freiburg, DE;
Mathias Knappenberger, Mietingen/Baltringen, DE;
Doreen Claussnitzer, Munich, DE;
Andreas Schaaf, Freiburg, DE;
Assignee:
GREENOVATION BIOTECH GMBH, Freiburg, DE;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/02 (2006.01); C12N 5/14 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12N 5/00 (2006.01); C12N 1/12 (2006.01); C12N 1/14 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C12N 1/12 (2013.01); C12N 1/14 (2013.01); C12N 5/0025 (2013.01); C12P 21/02 (2013.01); C12N 2500/60 (2013.01);
Abstract
The invention relates to a method for producing a recombinant protein in cells with a cell wall, comprising the step of increasing the secretion of the recombinant protein through the cell wall by expression of the protein in the cells in a culture medium containing a combination of a surface-active polymer and monovalent metal ions and with an osmolarity at least 0.32 osmol/L, said invention further relating to culture media and nutrient mixtures for the method.