The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 26, 2017

Filed:

Nov. 10, 2015
Applicant:

Laboratoire Francais Du Fractionnement ET Des Biotechnologies, Les Ulis, FR;

Inventors:

Gerard Perret, Choisy le Roi, FR;

Nicolas Bihoreau, Orsay, FR;

Laurent Siret, Villieres sur Orge, FR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 9/64 (2006.01); C07K 14/745 (2006.01); C12N 15/115 (2010.01);
U.S. Cl.
CPC ...
C12N 9/6432 (2013.01); C07K 14/745 (2013.01); C12N 9/644 (2013.01); C12N 9/6437 (2013.01); C12N 15/115 (2013.01); C12N 2310/16 (2013.01); C12N 2310/351 (2013.01); C12N 2330/31 (2013.01); C12Y 304/21006 (2013.01); C12Y 304/21021 (2013.01); C12Y 304/21022 (2013.01);
Abstract

The invention relates to a method for purifying biologically active GLA-domain coagulation proteins, comprising the following steps: a) bringing a sample that contains one or more GLA-domain coagulation proteins and may contain biologically inactive molecules of GLA-domain protein(s), into contact with an affinity support on which nucleic aptamers that bind specifically to at least one biologically active GLA-domain coagulation protein are immobilized, in order to form complexes between (i) said nucleic aptamers and (ii) said GLA-domain coagulation protein(s), b) releasing the GLA-domain coagulation protein(s) from the complexes formed in step a), and c) recovering said biologically active GLA-domain coagulation protein(s) in a purified form.


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