The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 19, 2017

Filed:

Feb. 28, 2014
Applicant:

Children's Medical Center Corporation, Boston, MA (US);

Inventors:

George Q. Daley, Weston, MA (US);

In-Hyun Park, Boston, MA (US);

Thorsten M. Schlaeger, Brookline, MA (US);

Elayne Chan, Auburndale, MA (US);

Sutheera Ratanasirintrawoot, Boston, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/536 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01); G01N 33/569 (2006.01); C12Q 1/68 (2006.01); G01N 33/577 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6872 (2013.01); C12Q 1/6881 (2013.01); G01N 33/5023 (2013.01); G01N 33/5073 (2013.01); G01N 33/56966 (2013.01); C12Q 2600/124 (2013.01); C12Q 2600/158 (2013.01); G01N 33/502 (2013.01); G01N 33/5044 (2013.01); G01N 33/5047 (2013.01); G01N 33/53 (2013.01); G01N 33/536 (2013.01); G01N 33/577 (2013.01); G01N 33/58 (2013.01); G01N 33/582 (2013.01); G01N 33/6854 (2013.01); G01N 2333/705 (2013.01); G01N 2333/70596 (2013.01); G01N 2333/90 (2013.01); G01N 2500/10 (2013.01);
Abstract

The methods and kits described herein are based, in part, to the discovery of a phenotype representing a fully-reprogrammed iPS cell and several reprogramming intermediates. The methods and kits described herein permit identification of fully-reprogrammed iPS cells and further permits one of skill in the art to monitor the emergence of iPS cells during the reprogramming process. The methods/kits can also be performed using real time using live cell imaging. Also described herein are methods for screening candidate reprogramming agents by monitoring the emergence of fully-reprogrammed iPS cells in the presence and absence of such an agent.


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