The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 19, 2017
Filed:
Sep. 23, 2014
National Taiwan University, Taipei, TW;
Shan-Hui Hsu, Taipei, TW;
Guo-Shiang Huang, Taipei, TW;
Niann-Tzyy Dai, Taipei, TW;
Lien-Guo Dai, Taipei, TW;
National Taiwan University, Taipei, TW;
Abstract
The present invention relates to a method of obtaining high purity stem cells from tissue, comprising: providing an impurity-containing cell mass obtained from a tissue; providing a filter device which comprises a cylinder structure, wherein the cylinder structure comprise an inlet and an outlet below and a content configured inside the cylinder structure between the inlet and the outlet; culturing the impurity-free cell mass on a polymeric film, wherein target stem cells of the impurity-free cell mass conjugate into a spheroid cell population; collecting the spheroid cell population from the polymeric film to obtain high purity target stem cells. According to the method of the present invention, stem cells can be rapidly and easily obtained from tissue. Only a small amount of tissue sample is required and the stem cells obtained can be readily used in clinical applications such as autotransplantation without the requirement of in vitro amplification.