The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 19, 2017
Filed:
Apr. 19, 2013
GE Healthcare Bio-sciences Ab, Uppsala, SE;
Cecilia Anneren, Uppsala, SE;
Christian Kaisermayer, Uppsala, SE;
Mats Lundgren, Uppsala, SE;
Johanna Tschop, Uppsala, SE;
Therese Lundstrom, Uppsala, SE;
Ann-Christin Magnusson, Uppsala, SE;
Gerald Blueml, Kapfenberg, AT;
GE Healthcare Bio-Sciences AB, Uppsala, SE;
Abstract
The present invention relates to a method for cell expansion. More closely, it relates to a method for expansion of cells, such as mesenchymal stem cells, on microcarriers in a plastic bag bioreactor. The invention enables expansion to therapeutic amounts of stem cells. The method comprises the following steps: a) addition of cells in cell culture medium and microcarriers to a plastic bag container; b) allowing the cells to adhere to the microcarriers while the container is kept substantially still; c) addition of further cell culture medium once the cells have adhered; d) culturing the cells under gentle and constant agitation; e) increase the surface area for continued culturing; and f) final harvesting of cells by an active detachment and separation step.