The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 19, 2017
Filed:
Jan. 28, 2015
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventor:
Christian Schantz, Penzberg, DE;
Assignee:
Hoffman-La Roche Inc., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12P 21/04 (2006.01); C12N 1/20 (2006.01); C07H 21/04 (2006.01); C07K 1/00 (2006.01); C07K 14/775 (2006.01); C12P 21/02 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/775 (2013.01); C12N 1/20 (2013.01); C12P 21/00 (2013.01); C12P 21/02 (2013.01);
Abstract
A method for cultivating a bacterial cell comprising the addition of an amino acid in an alkaline solution used for pH regulation. Also an aspect is a method for producing a polypeptide comprising the steps of a) providing a bacterial cell comprising a nucleic acid encoding the polypeptide, b) cultivating the provided cell, c) adjusting the pH value during the cultivating with a basic solution comprising an amino acid, d) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide.