The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 05, 2017
Filed:
Feb. 03, 2016
Federica Genovese, Frederiksberg, DK;
Mette Juul Nielsen, Copenhagen, DK;
Lise Larsen, Kastrup, DK;
Diana Julie Oersnes-leeming, Espergæde, DK;
Morten Karsdal, Copenhagen, DK;
Federica Genovese, Frederiksberg, DK;
Mette Juul Nielsen, Copenhagen, DK;
Lise Larsen, Kastrup, DK;
Diana Julie Oersnes-Leeming, Espergæde, DK;
Morten Karsdal, Copenhagen, DK;
Nordic Bioscience A/S, Herlev, DK;
Abstract
Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.