The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 05, 2017
Filed:
Jul. 03, 2015
Technische Universitaet Dresden, Dresden, DE;
Universitaet Leipzig, Leipzig, DE;
Roberta Galli, Dresden, DE;
Grit Preusse, Radebeul, DE;
Edmund Koch, Dresden, DE;
Gerald Steiner, Schwarzenberg, DE;
Maria-Elisabeth Krautwald-Junghanns, Markleeberg, DE;
Thomas Bartels, Gehrden, DE;
TECHNISCHE UNIVERSITAET DRESDEN, Dresden, DE;
UNIVERSITAET LEIPZIG, Leipzig, DE;
Abstract
The invention relates to a method for the Raman spectroscopic, in ovo sex determination of fertilized and hatched birds' eggs (), wherein the embryo, including the extra-embryonic structures, can move in the egg, and is not yet attached to the shell at the time of measuring. In addition, the following steps are carried out: monitoring the time course of the hatched egg until forming at least one recognizable blood vessel (); creating a hole () in the shell in the region near to the attached bloody vessel, using a hole-generating unit; finding the blood vessel forming in the egg, using a vision system () and a coaxial or lateral illumination with light () in the visible wavelength range; positioning at least one blood vessel in the laser focus of a laser source (), either by moving the egg or moving a lens () of a device () for introducing the laser light (), and detecting the Raman scattered radiation (); registering the Raman scattered radiation of the irradiated blood vessel using the device for introducing the laser light, and for detecting the Raman scattered radiation, wherein, during the measuring process, a movement of the blood vessel out of the focus can be avoided by tracking using the vision system; evaluating the Raman scattered radiation in an evaluation unit; determining and displaying the sex of the embryo in the bird's egg.