The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 05, 2017
Filed:
Jul. 31, 2014
Andreas Mershin, Cambridge, MA (US);
James Pelletier, Cambridge, MA (US);
Neil Gershenfeld, Cambridge, MA (US);
John Glass, Germantown, MD (US);
Elizabeth Strychalski, North Potomac, MD (US);
Andreas Mershin, Cambridge, MA (US);
James Pelletier, Cambridge, MA (US);
Neil Gershenfeld, Cambridge, MA (US);
John Glass, Germantown, MD (US);
Elizabeth Strychalski, North Potomac, MD (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
Abstract
In exemplary implementations, transplantation of nucleic acids into cells occurs in microfluidic chambers. The nucleic acids may be large nucleic acid molecules with more than 100 kbp. In some cases, the microfluidic chambers have only one orifice that opens to a flow channel. In some cases, flow through a microfluidic chamber temporarily ceases due to closing one or more valves. Transplantation occurs during a period in which the contents of the chambers are shielded from shear forces. Diffusion, centrifugation, suction from a vacuum channel, or dead-end loading may be used to move cells or buffers into the chambers.