The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 28, 2017
Filed:
Apr. 09, 2014
Base4 Innovation Ltd, Cambridge, Cambridgeshire, GB;
Medical Research Council, Swindon, Wiltshire, GB;
Cameron Alexander Frayling, Cambridgeshire, GB;
Barnaby Balmforth, Cambridgeshire, GB;
Bruno Flavio Nogueira de Sousa Soares, Cambridgeshire, GB;
Thomas Henry Isaac, Cambridgeshire, GB;
Boris Breiner, Cambridgeshire, GB;
Alessandra Natale, Cambridgeshire, GB;
Michele Amasio, Cambridgeshire, GB;
Paul Dear, Wiltshire, GB;
BASE4 INNOVATION LTD, Cambridge, GB;
MEDICAL RESEARCH COUNCIL, Wiltshire, GB;
Abstract
A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterized by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.