The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 28, 2017
Filed:
Nov. 30, 2012
Applicant:
Biolamina Ab, Sundbyberg, SE;
Inventors:
Karl Tryggvason, Djursholm, SE;
Kristian Tryggvason, Sundbyberg, SE;
Assignee:
BIOLAMINA AB, Sundbyberg, SE;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); C12N 5/00 (2006.01); C12N 5/02 (2006.01); C07K 14/705 (2006.01); C07K 14/78 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C07K 14/705 (2013.01); C07K 14/78 (2013.01); G01N 33/5091 (2013.01); G01N 2800/38 (2013.01);
Abstract
The present disclosure relates to the use of laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. Methods of enhancing genetic diagnosis by expanding a single stem cell on laminin-521 prior to PCR amplification are disclosed herein.