The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 28, 2017

Filed:

Sep. 18, 2013
Applicant:

General Electric Company, Schenectady, NY (US);

Inventors:

Nandini Nagraj, Clifton Park, NY (US);

Radislav Alexandrovich Potyrailo, Niskayuna, NY (US);

Andrew David Pris, Altamont, NY (US);

John Richard Nelson, Clifton Park, NY (US);

Assignee:

General Electric Company, Niskayuna, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C40B 30/04 (2006.01); C12N 15/10 (2006.01); G01N 33/53 (2006.01); H04N 1/32 (2006.01); H04N 1/40 (2006.01); H04N 1/60 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1034 (2013.01); C12N 15/1048 (2013.01); C12Q 1/6811 (2013.01); G01N 33/53 (2013.01); G01N 33/5308 (2013.01); H04N 1/3232 (2013.01); H04N 1/32309 (2013.01); H04N 1/32352 (2013.01); H04N 1/40 (2013.01); H04N 1/60 (2013.01); C40B 30/04 (2013.01); H04N 2201/0094 (2013.01); H04N 2201/327 (2013.01); H04N 2201/3233 (2013.01); H04N 2201/3271 (2013.01); H04N 2209/00 (2013.01);
Abstract

Methods for selecting a binding-element are provided. The method comprised of different steps. A first mixture is formed using at least one target molecule and a plurality of oligomers, followed by incubating the first mixture to form a second mixture comprising at least one target-bound oligomer and at least one target-unbound oligomer. Then a first accelerator is added to cleave the target-unbound oligomer and the target-bound oligomer is separated from the target molecule. This is followed by addition of a second accelerator for ligation, and a third accelerator for amplification followed by sequencing and post sequence analysis to select the binding-element.


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