The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 28, 2017
Filed:
Sep. 25, 2014
Applicant:
The University of North Carolina AT Chapel Hill, Chapel Hill, NC (US);
Inventors:
Darrel W. Stafford, Carrborro, NC (US);
Tao Li, San Diego, CA (US);
Assignee:
The University of North Carolina at Chapel Hill, Chapel Hill, NC (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/04 (2006.01); C12N 9/50 (2006.01); C12N 9/88 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0006 (2013.01); C12N 9/50 (2013.01); C12N 9/88 (2013.01); C12Q 1/6883 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/172 (2013.01); C12Y 101/04001 (2013.01); C12Y 304/21006 (2013.01); C12Y 401/0109 (2013.01);
Abstract
The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.