The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 2017
Filed:
Nov. 15, 2011
Menno Cornelis Van Zelm, Rotterdam, NL;
Jacobus Johannes Maria Van Dongen, Rotterdam, NL;
José Alberto Orfao DE Matos Correia E Vale, Salamanca, ES;
Menno Cornelis van Zelm, Rotterdam, NL;
Jacobus Johannes Maria van Dongen, Rotterdam, NL;
José Alberto Orfao de Matos Correia e Vale, Salamanca, ES;
ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM, Rotterdam, NL;
UNIERSIDAD DE SALAMANCA, Salamanca, ES;
Abstract
The invention relates to the field of medical diagnostics. In particular, it relates method and kits for identification and classification of IgE-related diseases, e.g. Type I hypersensitivity, as well as for monitoring of treatment efficacy, for instance anti-IgE therapy. Provided is a multi-color flow cytometric method for analyzing memory B cell and plasma cell subsets in a biological sample, comprising staining the sample with a panel of fluorochrome-conjugated antibodies comprising antibodies against IgM, IgA, IgG, IgD and IgE; an antibody against a B cell marker and an antibody against the CD38 antigen; subjecting the sample to flow cytometry and gating for lymphocytes based on forward scatter and side scatter pattern; gating the lymphocytes for expression of the B cell specific marker and CD38 to discriminate between CD38memory B cells and CD38plasma cells; and quantitating the IgE+ cells within the memory B cell population and/or the plasma cell population by the negative selection of cells expressing IgM, IgA, IgG and/or IgD and the positive selection of IgE expressing cells.