The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 21, 2017

Filed:

Mar. 14, 2014
Applicant:

Nugen Technologies, Inc., San Carlos, CA (US);

Inventors:

Doug Amorese, Los Altos, CA (US);

Benjamin G. Schroeder, San Mateo, CA (US);

Jonathan Scolnick, San Francisco, CA (US);

Assignee:

Nugen Technologies, Inc., San Carlos, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G06F 19/22 (2011.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); G06F 19/22 (2013.01);
Abstract

Described herein are improved methods, compositions and kits for next generation sequencing (NGS). The methods, compositions and kits described herein enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (which can comprise regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information can be obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein can be useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.


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