The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 2017
Filed:
Mar. 07, 2014
Bio-rad Laboratories, Inc., Hercules, CA (US);
Adam Lowe, Mountain House, CA (US);
Geoff McDermott, Pleasanton, CA (US);
Christopher M. Hindson, Pleasanton, CA (US);
Erin R. Chia, Berkeley, CA (US);
Amy L. Hiddessen, Dublin, CA (US);
Benjamin J. Hindson, Livermore, CA (US);
Chunxiao Han, Dublin, CA (US);
Yaqi Wang, Dublin, CA (US);
Bio-Rad Laboratories, Inc., Hercules, CA (US);
Abstract
The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.