The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2017
Filed:
Jan. 28, 2014
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, CN;
Xueli Zhang, Tianjin, CN;
Qingyan Li, Tianjin, CN;
Jing Zhao, Tianjin, CN;
Tao Sun, Tianjin, CN;
Guanping Dai, Tianjin, CN;
Hongtao Xu, Tianjin, CN;
Jinlei Tang, Tianjin, CN;
Yanhe Ma, Tianjin, CN;
Abstract
Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of α-ketoglutarate dehydrogenase inor the mutant thereof. The method for improving the enzymatic activity of α-ketoglutarate dehydrogenase inor the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) inor the mutant thereof with any of the following regulating elements: artificial regulating element M1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of α-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved.