The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 07, 2017
Filed:
Feb. 22, 2012
Applicants:
Jin Soo Kim, Seoul, KR;
Seok Joong Kim, Seoul, KR;
Yong Sub Kim, Busanjin-gu, KR;
Inventors:
Assignee:
TOOLGEN INC., Seoul, KR;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 33/50 (2006.01); C12N 15/64 (2006.01); C12Q 1/44 (2006.01); C12Q 1/02 (2006.01); C12Q 1/66 (2006.01); C12N 15/63 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/44 (2013.01); C12N 15/64 (2013.01); G01N 33/50 (2013.01);
Abstract
The present invention relates to a reporter construct and method for identifying or enriching the cells, wherein a specific endogenous nucleotide sequence is cleaved by a specific nuclease or modified by such cleavage; a host cell comprising the reporter construct; and a system for monitoring a nuclease activity. The reporter system of the present invention is simple and non-invasive, and allows for an efficient enrichment of the gene-modified cells. Therefore, the present invention will promote the application of a nuclease in the field of gene therapy and genetic engineering as well as basic research.