The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 07, 2017

Filed:

Oct. 12, 2015
Applicant:

Allosource, Centennial, CO (US);

Inventor:

Yaling Shi, Larkspur, CO (US);

Assignee:

AlloSource, Centennial, CO (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 35/38 (2015.01); A61L 27/38 (2006.01); A61L 27/36 (2006.01); A61L 27/54 (2006.01); C12N 5/0775 (2010.01); A01N 1/02 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
A61L 27/3834 (2013.01); A01N 1/021 (2013.01); A61K 35/38 (2013.01); A61L 27/3608 (2013.01); A61L 27/3847 (2013.01); A61L 27/3895 (2013.01); A61L 27/54 (2013.01); C12N 5/0662 (2013.01); C12N 5/0667 (2013.01); A61K 35/12 (2013.01); A61L 2300/406 (2013.01); A61L 2430/02 (2013.01); C12N 2509/00 (2013.01); C12N 2533/18 (2013.01); C12N 2533/90 (2013.01);
Abstract

There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose tissue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the substrate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove unwanted cells from the substrate. Other embodiments are also disclosed.


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