The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 07, 2017
Filed:
Mar. 28, 2016
Rui Hou, Wuhan, CN;
Yuwei Wang, Wuhan, CN;
Huifang Liang, Wuhan, CN;
Zhanguo Zhang, Wuhan, CN;
Zhimin Liu, Wuhan, CN;
Binhao Zhang, Wuhan, CN;
Bixiang Zhang, Wuhan, CN;
Xiaoping Chen, Wuhan, CN;
Rui Hou, Wuhan, CN;
Yuwei Wang, Wuhan, CN;
Huifang Liang, Wuhan, CN;
Zhanguo Zhang, Wuhan, CN;
Zhimin Liu, Wuhan, CN;
Binhao Zhang, Wuhan, CN;
Bixiang Zhang, Wuhan, CN;
Xiaoping Chen, Wuhan, CN;
Other;
Abstract
A method for establishing an animal model of hepatocellular carcinoma (HCC) bone metastasis, the method including: 1) establishing 97H and LM3 cell clones with stable expression of firefly luciferase (LUC); 2) allowing HCC cells to form bone metastasis in nude mice via intratibial injection; 3) reproducing HCC bone metastasis in nude mice via intracardiac injection of tumor cells; and 4) isolating a sub-population of tumor cells that targets metastasis to bone. The 97H and the LM3 are highly metastatic HCC cell lines transfected with luciferase gene. BALB/cA-nu mice are 4-5 weeks old and maintained in laminar flow cabinets under SPF conditions and received human care throughout an entire study. A cell number for intratibial injection is 0.5×10, and a cell number for intracardiac injection is 1×10.
Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile