The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 24, 2017
Filed:
Jan. 12, 2015
Applicant:
Uab Baltymas, Vilnius, LT;
Inventors:
Evaldas Ciplys, Vilnius, LT;
Rimantas Slibinskas, Vilnius, LT;
Kestutis Sasnauskas, Vilnius, LT;
Marek Michalak, Edmonton, CA;
Leslie Ina Gold, Darien, CT (US);
Assignee:
UAB BALTYMAS, Vilnius, LT;
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/90 (2006.01); C07K 14/47 (2006.01); C12N 15/81 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/90 (2013.01); C07K 14/47 (2013.01); C07K 14/4702 (2013.01); C07K 14/4728 (2013.01); C12N 15/81 (2013.01); C12P 21/02 (2013.01); C12Y 503/04001 (2013.01);
Abstract
A method using yeast as a host for production of human ER chaperone proteins, using endogenous signal peptides of intracellular human proteins that are recognized and correctly processed in the yeast cells to subsequently lead to the secretion of the human proteins. The resultant proteins possessed native amino acid sequence and were biologically active. Moreover, secretion allowed simple one-step purification of native recombinant human proteins with high yields.