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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9796971 B1

PDFFull Text
Date of Patent:
Oct. 24, 2017

Filed:

Jan. 12, 2015

Generation of native recombinant secreted human endoplasmic reticulum chaperones by using their native signal sequences in yeast expression systems

Applicant:

Uab Baltymas, Vilnius, LT;

Inventors:

Evaldas Ciplys, Vilnius, LT;

Rimantas Slibinskas, Vilnius, LT;

Kestutis Sasnauskas, Vilnius, LT;

Marek Michalak, Edmonton, CA;

Leslie Ina Gold, Darien, CT (US);

Assignee:

UAB BALTYMAS, Vilnius, LT;

Attorneys:

Shelly Guest Cermak

Cermak Nakajima & McGowan LLP

Primary Examiner:

Maria Leavitt

Int. Cl.
CPC ...
C12N 9/90 (2006.01); C07K 14/47 (2006.01); C12N 15/81 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/90 (2013.01); C07K 14/47 (2013.01); C07K 14/4702 (2013.01); C07K 14/4728 (2013.01); C12N 15/81 (2013.01); C12P 21/02 (2013.01); C12Y 503/04001 (2013.01);
Abstract

A method using yeast as a host for production of human ER chaperone proteins, using endogenous signal peptides of intracellular human proteins that are recognized and correctly processed in the yeast cells to subsequently lead to the secretion of the human proteins. The resultant proteins possessed native amino acid sequence and were biologically active. Moreover, secretion allowed simple one-step purification of native recombinant human proteins with high yields.

Find Patent Forward Citations

Ciplys, Evaldas. (2017). Generation of native recombinant secreted human endoplasmic reticulum chaperones by using their native signal sequences in yeast expression systems (U.S. Patent No. 9796971). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9796971

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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