The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 17, 2017

Filed:

Mar. 13, 2014
Applicants:

Marc P. Raphael, Washington, DC (US);

Joseph A. Christodoulides, Alexandria, VA (US);

Jeff M. Byers, Fairfax Station, VA (US);

James B. Delehanty, Washington, DC (US);

Inventors:

Marc P. Raphael, Washington, DC (US);

Joseph A. Christodoulides, Alexandria, VA (US);

Jeff M. Byers, Fairfax Station, VA (US);

James B. Delehanty, Washington, DC (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/551 (2006.01); G01N 21/552 (2014.01); G01N 33/543 (2006.01); G01N 21/64 (2006.01); G01N 21/59 (2006.01); G01N 21/27 (2006.01);
U.S. Cl.
CPC ...
G01N 21/554 (2013.01); G01N 21/59 (2013.01); G01N 21/648 (2013.01); G01N 21/6458 (2013.01); G01N 33/54373 (2013.01); G01N 21/274 (2013.01); G01N 2021/6491 (2013.01);
Abstract

A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.


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