The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 10, 2017

Filed:

Aug. 07, 2013
Applicants:

Commissariat a L'energie Atomique ET Aux Energies Alternatives, Paris, FR;

Centre National DE LA Recherche Scientifique, Paris, FR;

Inventors:

Michael Plevin, Grenoble, FR;

Rime Kerfah, Fontaine, FR;

Ombeline Pessey, Fontaine, FR;

Jérôme Boisbouvier, Saint Vincent de Mercuze, FR;

Pierre Gans, Voreppe, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); C07C 59/215 (2006.01); C07B 59/00 (2006.01); C12P 21/02 (2006.01); C07K 1/13 (2006.01); C12P 7/42 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/58 (2013.01); C07B 59/001 (2013.01); C07C 59/215 (2013.01); C07K 1/13 (2013.01); C12P 7/42 (2013.01); C12P 21/02 (2013.01); G01N 33/6812 (2013.01); G01N 2458/15 (2013.01);
Abstract

The present invention relates to labeled chiral alpha-hydroxy ketoacid derivatives, a process for preparing the derivatives and their use for isotopic labeling of amino acids, in particular, for isotopic labeling of methyl groups of amino acids, and more particularly, for specific isotopic labeling of valine, leucine and isoleucine methyl groups, in proteins and biomolecular assemblies. The invention also concerns a process for analyzing proteins and biomolecular assemblies by NMR spectroscopy including a step of isotopic labeling of amino acids, in particular, valine, leucine and isoleucine, in proteins and biomolecular assemblies to be analyzed by the chiral alpha-hydroxy ketoacid derivatives of the invention. The invention further relates to a kit for isotopic labeling of valine, leucine and isoleucine amino acids, in proteins and biomolecular assemblies including one or more chiral alpha-hydroxy ketoacid derivatives of the invention.


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