The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 10, 2017

Filed:

Dec. 29, 2015
Applicant:

Flagship Biosciences, Inc., Westminster, CO (US);

Inventors:

Anthony J. Milici, Branford, CT (US);

George David Young, Ridgway, CO (US);

Holger Lange, Enger, DE;

Joseph Krueger, Andover, MA (US);

Nathan T. Martin, Boulder, CO (US);

Assignee:

Flagship Biosciences, Inc., Westminster, CO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G06K 9/00 (2006.01); G01N 15/14 (2006.01); G01N 1/30 (2006.01); G01N 33/68 (2006.01); G06T 7/00 (2017.01); G01N 15/10 (2006.01); G01N 15/00 (2006.01);
U.S. Cl.
CPC ...
G01N 15/1463 (2013.01); G01N 1/30 (2013.01); G01N 33/6887 (2013.01); G06K 9/00147 (2013.01); G06T 7/0012 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1465 (2013.01); G01N 2015/1497 (2013.01); G01N 2333/4712 (2013.01); G01N 2800/10 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/30004 (2013.01);
Abstract

The disclosure concerns a method for assessing muscular dystrophy-linked protein expression in muscle fibers using digital image analysis of tissue. The method relates to assessing disease severity in individuals with muscular dystrophy. Muscle tissue samples are obtained from patients submitted for evaluation and processed to produce tissue sections mounted on glass slides which have been stained for a muscular dystrophy-linked protein. Digital images of the stained tissue sections are generated and analyzed by applying an algorithm process implemented by a computer to the images. The algorithm process extracts the morphometric and staining features of the muscular dystrophy-linked protein staining in the tissue, and parameters relating to these features are used to score the disease status for each patient submitted for evaluation. The score of disease status is ultimately used to infer disease severity, monitor the efficacy of a therapeutic approach, or select patients as candidates for a therapeutic approach.


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