The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 10, 2017
Filed:
Oct. 18, 2016
King Saud University, Riyadh, SA;
Periasamy Vaiyapuri Subbarayan, Riyadh, SA;
Ali Abdullah Alshatwi, Riyadh, SA;
Jegan Athinarayanan, Riyadh, SA;
KING SAUD UNIVERSITY, Riyadh, SA;
Abstract
The method of making a three-dimensional, leaf-based scaffold for three-dimensional cell cultures includes washing a quantity ofleaves, then treating the washedleaves in a sodium hydroxide solution to obtain alkali-treatedleaves. The alkali-treatedleaves are washed, and then superficial tissue is removed from the alkali-treatedleaves to obtainleaf skeletons. Theleaf skeletons are dried and then consecutively immersed in distilled water, a phosphate buffer saline solution, and plain Dulbecco's modified Eagle's medium (DMEM) to form the three-dimensional scaffolds for three-dimensional cell cultures. Each three-dimensional scaffold can be used for growing three-dimensional cell cultures, such as human mesenchymal stem cell cultures.