The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 03, 2017
Filed:
Jun. 29, 2012
John Richard Nelson, Clifton Park, NY (US);
Robert Scott Duthie, Schenectady, NY (US);
Gregory Andrew Grossmann, Halfmoon, NY (US);
Ryan Charles Heller, Guilderland, NY (US);
John Richard Nelson, Clifton Park, NY (US);
Robert Scott Duthie, Schenectady, NY (US);
Gregory Andrew Grossmann, Halfmoon, NY (US);
Ryan Charles Heller, Guilderland, NY (US);
General Electric Company, Niskayuna, NY (US);
Abstract
A method of amplifying RNA template is provided. The method comprises reverse-transcribing a ribonucleic acid (RNA) template to form a cDNA using a first reaction mixture comprising RNA template, at least one primer capable of hybridizing to the RNA template, a reverse transcriptase and deoxynucleoside triphosphates (dNTPs); and amplifying the cDNA to form an amplified product using a second reaction mixture comprising at least one strand displacement DNA polymerase, at least one inosine-containing primer and a nuclease that is capable of nicking DNA 3' to an inosine residue of the primer. The method is accomplished under an isothermal condition without denaturing the cDNA template. A method of quantifying RNA template in a sample and a method of detecting RNA template in a sample are also provided.