The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 26, 2017

Filed:

Sep. 12, 2014
Applicant:

The University of Queensland, St. Lucia, Queensland, AU;

Inventors:

Viktor Stein, St. Lucia, AU;

Kirill Alexandrov, St. Lucia, AU;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/542 (2006.01); C07K 19/00 (2006.01); C12N 9/48 (2006.01); C07K 14/00 (2006.01); C07K 14/005 (2006.01); C07K 14/47 (2006.01); C07K 16/00 (2006.01); C12N 7/00 (2006.01); C12N 9/50 (2006.01); C12N 9/90 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/542 (2013.01); C07K 14/00 (2013.01); C07K 14/005 (2013.01); C07K 14/4728 (2013.01); C07K 16/00 (2013.01); C07K 19/00 (2013.01); C12N 7/00 (2013.01); C12N 9/48 (2013.01); C12N 9/506 (2013.01); C12N 9/90 (2013.01); G01N 33/582 (2013.01); C07K 2319/50 (2013.01); C07K 2319/61 (2013.01); C07K 2319/70 (2013.01); C12N 2770/34011 (2013.01); C12Y 304/22044 (2013.01); C12Y 502/01008 (2013.01); G01N 2333/08 (2013.01); G01N 2333/185 (2013.01); G01N 2333/4727 (2013.01); G01N 2333/9513 (2013.01);
Abstract

A biosensor comprises first and second molecular components and is capable of displaying protease activity in response to a binding event mediated by first and second binding partners of the biosensor. The first and second binding partners may bind each other directly or may both bind a target molecule. At least the first molecular component comprises an autoinhibited protease, whereby the binding event switches the protease frora an autoinhibited inactive state to a protease active state. The second molecular component may activate the protease of the first molecular component by binding a cross-binder which releases the autoinhibitor or by cleaving a linker which releases the autoinhibitor. The first and second molecular components may both have autoinhibited proteases which reciprocally activate each other.


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