The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 26, 2017
Filed:
Jun. 19, 2015
Applicant:
Agilent Technologies, Inc., Santa Clara, CA (US);
Inventors:
Alexander Belyaev, San Diego, CA (US);
Nicholas M. Sampas, San Jose, CA (US);
Alicia Scheffer-Wong, San Jose, CA (US);
Assignee:
Agilent Technologies, Inc., Santa Clara, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12N 15/10 (2006.01); C12N 9/12 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12N 15/1065 (2013.01); C12Q 2521/327 (2013.01); C12Q 2563/179 (2013.01);
Abstract
The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.