The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 26, 2017

Filed:

Aug. 05, 2015
Applicant:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Inventors:

James A. Thomson, Madison, WI (US);

Zhonggang Hou, Shrewsbury, MA (US);

Guokai Chen, Madison, WI (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/02 (2006.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01); C07K 14/78 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); C07K 14/78 (2013.01); C12N 5/0696 (2013.01); C12N 2500/02 (2013.01); C12N 2500/05 (2013.01); C12N 2500/25 (2013.01); C12N 2500/38 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01); C12N 2501/33 (2013.01); C12N 2501/727 (2013.01); C12N 2533/50 (2013.01); C12N 2533/52 (2013.01);
Abstract

Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented. Also provided herein are defined culture systems for maintaining human pluripotent stem cells in a substantially undifferentiated state, where the defined culture system comprises human pluripotent stem cells, a defined culture medium, and at least one polypeptide selected from the group consisting of residues 43 to 378 of SEQ ID NO:1 and residues 45 to 378 of SEQ ID NO:1, and where the defined culture medium comprises insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEM/F12, NaHCO, and one of TGFβ and NODAL.


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Thomson, James. (2017). Vitronectin-derived cell culture substrate for maintaining human pluripotent stem cells in a substantially undifferentiated state (U.S. Patent No. 9771556). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9771556

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