The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 19, 2017

Filed:

May. 29, 2014
Applicant:

Gnubio, Inc., Cambridge, MA (US);

Inventors:

David H. Tracy, Champaign, IL (US);

Aaron Weber, Arlington, MA (US);

Peter Stokes, Cambridge, MA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01P 5/20 (2006.01); G01P 5/00 (2006.01); G01F 1/708 (2006.01); G01N 15/02 (2006.01); B01L 3/00 (2006.01); G01F 22/00 (2006.01); G01N 35/08 (2006.01); G01N 15/14 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
G01P 5/20 (2013.01); B01L 3/502715 (2013.01); B01L 3/502784 (2013.01); G01F 1/7086 (2013.01); G01F 22/00 (2013.01); G01N 15/0227 (2013.01); G01N 15/1434 (2013.01); G01N 15/1459 (2013.01); G01N 15/1463 (2013.01); G01N 15/1484 (2013.01); G01P 5/001 (2013.01); B01L 2200/143 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/082 (2013.01); G01N 35/08 (2013.01); G01N 2015/1075 (2013.01); G01N 2015/1493 (2013.01);
Abstract

Systems and methods are provided for determining a velocity or an inflation rate of a droplet in a microfluidic channel. The droplet is exposed to two or more temporally separated flashes of light, each flash including light of one wavelength band, and imaged using a detector configured to distinguish light in the wavelength bands. Two or more images of the droplet are acquired, each corresponding to one of the flashes, and all within a single video frame or photographic exposure. The images can be processed separately and the position or size of the droplet in each image is calculated. A velocity or inflation rate is then determined by dividing the change in position or size by the amount of time allowed to pass between the flashes.


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