The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 2017
Filed:
Feb. 23, 2015
Applicant:
Oncu Limited, Tortola, VG;
Inventors:
Christofer Toumazou, London, GB;
Melpomeni Kalofonou, London, GB;
Assignee:
ONCU LIMITED, Tortola, VG;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12Q 1/68 (2006.01); G01N 27/00 (2006.01); G01N 27/30 (2006.01); G01N 27/26 (2006.01); G01N 27/403 (2006.01); G01N 27/414 (2006.01);
U.S. Cl.
CPC ...
G01N 27/4145 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6858 (2013.01); C12Q 1/6869 (2013.01);
Abstract
The use of ion sensitive field effect transistor (ISFET) to detect methylated nucleotides in a DNA sample is described. A method of detecting methylated nucleotides in a DNA sample may include the steps of treating a sample of DNA with a reagent which discriminates between methylated and non-methylated nucleotides to provide treated DNA, amplifying the treated DNA and optionally sequencing the amplified DNA. An ISFET is used to monitor the addition of one or more dNTPs in the strand extension reactions during the amplification and/or sequencing step. Suitable apparatus is also provided.