The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 2017
Filed:
Sep. 03, 2014
Dow Agrosciences Llc, Indianapolis, IN (US);
Lakshmi Sastry-Dent, Avon, IN (US);
W. Michael Ainley, Carmel, IN (US);
Jayakumar P. Samuel, Carmel, IN (US);
Zehui Cao, Westfield, IN (US);
Liu Y. Shen, Westfield, IN (US);
Cristie M. Dewes, Indianapolis, IN (US);
Dow AgroSciences LLC, Indianapolis, IN (US);
Abstract
The present disclosure provides methods for detecting and identifying plant events that contain donor sequences inserted precisely into targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce a first amplicon from donor sequences inserted in the reverse orientation, amplifying the first amplicon with a second round of PCR and detecting the presence of the second amplicon, wherein the production of the first and second amplicon indicates the presence of the site specific integration event.