The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 12, 2017

Filed:

Jun. 17, 2011
Applicants:

Yoko Mullen, Sherman Oaks, CA (US);

Masato Mitsuhashi, Irvine, CA (US);

Keiko Omori, Pasadena, CA (US);

Inventors:

Yoko Mullen, Sherman Oaks, CA (US);

Masato Mitsuhashi, Irvine, CA (US);

Keiko Omori, Pasadena, CA (US);

Assignees:

City of Hope, Duarte, CA (US);

Hitachi Chemical Research Center, Inc., Irvine, CA (US);

Hitachi Chemical Co., Ltd., Tokyo, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6883 (2013.01); G01N 33/507 (2013.01); C12Q 2600/136 (2013.01); C12Q 2600/158 (2013.01); G01N 2333/4748 (2013.01); G01N 2333/525 (2013.01);
Abstract

p53-upregulated modulator of apoptosis (PUMA) is a biomarker associated with islet cell health. If PUMA is low, islet cells are typically healthy. If PUMA is high, islet cells are typically unhealthy or dying. PUMA may be measured by either measuring its nucleic or amino acid. PUMA mRNA may be induced by TNF-α stimulation in a time- and dose-dependent manner and β cell apoptosis is induced through a mitochondrial pathway. TNF-α significantly inhibited glucose-induced preproinsulin precursor mRNA synthesis. Such β cell stress signaling in human islets indicates overall state of islet health and, ultimately, the risk of onset and/or degree of severity of both type 1 and type 2 diabetes mellitus.


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