The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 12, 2017

Filed:

Oct. 28, 2015
Applicant:

Wafergen, Inc., Fremont, CA (US);

Inventors:

Syed A. Husain, Fremont, CA (US);

Bradley L. Griswold, Fremont, CA (US);

Michael Slater, Fremont, CA (US);

Patricio A. Espinoza Vallejos, Fremont, CA (US);

Jude Dunne, Fremont, CA (US);

Glenn Hein, Fremont, CA (US);

Maithreyan Srinivasan, Fremont, CA (US);

Assignee:

Takara Bio USA, Inc., Mountain View, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B01J 19/00 (2006.01); C12M 1/34 (2006.01); B01L 3/00 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
B01J 19/0046 (2013.01); B01L 3/50857 (2013.01); B01L 3/502761 (2013.01); B01J 2219/00527 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00709 (2013.01); B01J 2219/00718 (2013.01); B01L 2200/025 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/165 (2013.01); B01L 2400/049 (2013.01); G01N 1/405 (2013.01);
Abstract

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and/or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bottom of each dimple or well of the capture chip has a positive electrical charge sufficient to attract cells from a cell suspension flowing over the dimples or wells.


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