The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 05, 2017
Filed:
Nov. 30, 2011
Yasuhiro Fujino, Osaka, JP;
Risako Fujita, Osaka, JP;
Kouichi Wada, Osaka, JP;
Kotomi Oda, Osaka, JP;
Takuya Ueda, Tokyo, JP;
Yoshihiro Shimizu, Tokyo, JP;
Takashi Kanamori, Tokyo, JP;
Yasuhiro Fujino, Osaka, JP;
Risako Fujita, Osaka, JP;
Kouichi Wada, Osaka, JP;
Kotomi Oda, Osaka, JP;
Takuya Ueda, Tokyo, JP;
Yoshihiro Shimizu, Tokyo, JP;
Takashi Kanamori, Tokyo, JP;
Mitsubishi Tanabe Pharma Corporation, Osaka, JP;
The University of Tokyo, Tokyo, JP;
Abstract
The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3'-end a structure necessary for maintaining a complex with the Fab encoded by itself; and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.