The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 05, 2017

Filed:

Aug. 28, 2014
Applicant:

Abbott Point of Care Inc., Princeton, NJ (US);

Inventors:

Gordon Bruce Collier, Fitzroy Harbour, CA;

John Allister Wood, Kanata, CA;

Jason Andrew MacLeod, Ottawa, CA;

William Charles Dicke, Ottawa, CA;

Attila Csaba Nemeth, Ottawa, CA;

Cary James Miller, Ottawa, CA;

Assignee:

Abbott Point of Care Inc., Princeton, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); B01L 3/502769 (2013.01); B01L 7/52 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6844 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0816 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0487 (2013.01); C12N 15/1013 (2013.01);
Abstract

The present invention relates to methods for the extraction of nucleic acids from cells, the amplification of segments of nucleic acid and the detection of nucleic acids, all in a convenient and portable manner. In one embodiment, a sample comprising cells containing nucleic acid is exposed to an aqueous mixture comprising a lytic reagent and one or more beads capable of binding the nucleic acid released from said cells to form a nucleic acid-bead complex. The nucleic acid-bead complex is passed through an immiscible liquid layer to separate the nucleic acid from the aqueous mixture. The one or more beads are magnetic, and the nucleic acid-bead complex is passed through and separated from the immiscible liquid layer with an applied magnetic field. The invention is particularly suited for use in point-of-care medical diagnostics testing.


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