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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9752116 B1

PDFFull Text
Date of Patent:
Sep. 05, 2017

Filed:

Aug. 12, 2016

Self-assembling cell aggregates and methods of making engineered tissue using the same

Applicants:

The Curators of the University of Missouri, Columbia, MO (US);

Musc Foundation for Research Development, Charleston, SC (US);

Inventors:

Gabor Forgacs, Potsdam, NY (US);

Karoly Jakab, Columbia, MO (US);

Adrian Neagu, Columbia, MO (US);

Vladimir Mironov, Mount Pleasant, SC (US);

Assignees:

The Curators of the University of Missouri, Columbia, MO (US);

MUSC Foundation for Research Development, Charleston, SC (US);

Attorney:

Senniger Powers LLP

Primary Examiner:

Debbie K Ware

Assistant Examiner:

Kailash C Srivastava

Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/07 (2010.01); C12N 11/00 (2006.01); C12N 11/12 (2006.01); C12N 5/071 (2010.01); C12M 1/26 (2006.01); B29C 67/00 (2017.01); C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/36 (2006.01); B33Y 70/00 (2015.01); B33Y 10/00 (2015.01); B33Y 50/02 (2015.01); B33Y 30/00 (2015.01); B33Y 80/00 (2015.01); B29L 31/00 (2006.01); B29K 105/00 (2006.01); B29K 33/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); B29C 67/0059 (2013.01); B29C 67/0088 (2013.01); B33Y 10/00 (2014.12); B33Y 50/02 (2014.12); B33Y 70/00 (2014.12); C12M 21/08 (2013.01); C12M 33/12 (2013.01); C12M 41/48 (2013.01); C12M 47/00 (2013.01); C12N 5/0697 (2013.01); B29K 2033/26 (2013.01); B29K 2105/0035 (2013.01); B29K 2105/0061 (2013.01); B29L 2031/7532 (2013.01); B33Y 30/00 (2014.12); B33Y 80/00 (2014.12);
Abstract

A composition comprising a plurality of cell aggregates for use in the production of engineered organotypic tissue by organ printing. A method of making a plurality of cell aggregates comprises centrifuging a cell suspension to form a pellet, extruding the pellet through an orifice, and cutting the extruded pellet into pieces. Apparatus for making cell aggregates comprises an extrusion system and a cutting system. In a method of organ printing, a plurality of cell aggregates are embedded in a polymeric or gel matrix and allowed to fuse to form a desired three-dimensional tissue structure. An intermediate product comprises at least one layer of matrix and a plurality of cell aggregates embedded therein in a predetermined pattern. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type, matrix, and embedding patterns to enable selection of organ printing processes parameters for use in producing an engineered tissue having a desired three-dimensional structure.

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