The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 29, 2017

Filed:

Nov. 19, 2014
Applicant:

Roche Molecular Systems, Inc., Pleasanton, CA (US);

Inventors:

Jenny A Johnson, Castro Valley, CA (US);

Stephen G Will, Oakland, CA (US);

Assignee:

Roche Molecular Systems, Inc., Pleasanton, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12N 15/11 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6818 (2013.01); C07H 21/00 (2013.01); C12Q 1/6832 (2013.01); C12Q 2561/101 (2013.01);
Abstract

Photoblocked probes are disclosed including a specific hydrolysis probe having a first nucleic acid sequence complementary to a target having a second nucleic acid sequence, a first and a second interactive labels, a 5' end and a 3′ end, and one or more photocleavable moieties coupled to one or more nucleotides of the specific hydrolysis probe, wherein the photocleaveable moiety interferes with the hybridization of the specific hydrolysis probe with the region of the amplification product. Also disclosed are PCR methods for the detection of the presence or absence of a target nucleic acid in a sample utilizing the photoblocked probes, as well as kits.


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