The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 29, 2017
Filed:
May. 17, 2012
Robert Terbrueggen, Manhattan Beach, CA (US);
Yenbou Liu, Arcadia, CA (US);
John Ray Childers, Jr., Long Beach, CA (US);
Chang Hee Kim, Rancho Palos Verdes, CA (US);
Majid R. Abedi, Anaheim, CA (US);
Robert Terbrueggen, Manhattan Beach, CA (US);
Yenbou Liu, Arcadia, CA (US);
John Ray Childers, Jr., Long Beach, CA (US);
Chang Hee Kim, Rancho Palos Verdes, CA (US);
Majid R. Abedi, Anaheim, CA (US);
DXTERITY DIAGNOSTICS INCORPORATED, Rancho Dominguez, CA (US);
Abstract
The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.