The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 29, 2017
Filed:
Nov. 04, 2013
Applicant:
Discuva Limited, Cambridge, GB;
Inventors:
David Hugh Williams, Cambridge, GB;
Arthur Keith Turner, Cambridge, GB;
Assignee:
DISCUVA LIMITED, Cambridge, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 1/21 (2006.01); C40B 30/06 (2006.01); G06F 19/18 (2011.01);
U.S. Cl.
CPC ...
C12N 15/1082 (2013.01); C12N 15/102 (2013.01); C40B 30/06 (2013.01); G06F 19/18 (2013.01);
Abstract
Provided are methods for identifying differentially represented genes, the method comprising the steps of generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (Tn), wherein the Tncomprises a promoter such that transposon insertion into bacterial DNA increases the transcription of a gene at or near the insertion site; growing bacteria from the mutant pool in the presence of different amounts of said antibiotic to produce two or more test cultures; and comparing the distribution of Tninsertions between test cultures.