The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 22, 2017

Filed:

Mar. 15, 2013
Applicant:

Mbio Diagnostics, Inc., Boulder, CO (US);

Inventors:

Kevin D. Moll, Boulder, CO (US);

Marie J. Delaney, Boulder, CO (US);

Kurt R. Vogel, Boulder, CO (US);

Michael J. Lochhead, Boulder, CO (US);

Jeffrey Ives, Arvada, CO (US);

John S. Dunn, Arvada, CO (US);

Keagan B. Rowley, Boulder, CO (US);

Assignee:

MBio Diagnostics, Inc., Boulder, CO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 3/00 (2006.01); G01N 21/64 (2006.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6428 (2013.01); B01L 3/502723 (2013.01); G01N 21/6456 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/12 (2013.01); B01L 2200/16 (2013.01); B01L 2300/044 (2013.01); B01L 2300/045 (2013.01); B01L 2300/046 (2013.01); B01L 2300/048 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/161 (2013.01); B01L 2400/0406 (2013.01);
Abstract

A particle identification system includes: a cartridge for containing a sample with fluorescently labeled particles; illumination for illuminating a region within the cartridge to stimulate emission from particles; imager for generating wavelength-filtered electronic images of the emission within at least one measurement field of the region; and particle identifier for processing the electronic images to determine a superset of particles of interest, and fluorescently labeled particles within the superset based on properties of the particles in the at least one measurement field. A method determines fluorescently labeled particles within a sample, by: processing at least one electronic image from at least one focal position within the sample; determining dimmest separation lines between brighter areas in the electronic image; and, for each of the brighter areas, determining local background level based on pixel values of the separation lines forming a perimeter therearound, to determine each of the fluorescently labeled particles.


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