The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 22, 2017

Filed:

Oct. 26, 2016
Applicants:

Ecole Normale Superieur, Paris, FR;

Centre National DE LA Recherche Scientifique (Cnrs), Paris, FR;

Universite Pierre ET Marie Curie (Paris 6), Paris, FR;

Inventors:

David Bensimon, Paris, FR;

Vincent Croquette, Antony, FR;

Jean-Francois Allemand, Arcueil, FR;

Maria Manosas, Barcelona, ES;

Fang-Yuan Ding, Paris, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01);
Abstract

The present invention relates to a method for the determination of a nucleic acid sequence by physical manipulation. The method is based on the precise determination of the localization of the replicating fork on the template by measuring the physical distance between one end of the molecule and the fork. This allows the determination of the physical location of the site where a pause or a blockage of the replication occurs, and deducing therefrom information on the sequence of the nucleic acid.


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